Local intragraft humoral immune responses in chronic lung allograft dysfunction.
Evidence level: human tissue laboratory study (15 CLAD patients); abstract-only
A Toronto laboratory study of lung tissue from 15 CLAD patients, reporting that donor-specific and non-HLA antibodies can be produced within the lung allograft.
As of . Primary source: Publisher record via DOI.
Summary
According to the abstract, lung tissue was prospectively collected from 15 CLAD patients undergoing retransplantation or autopsy and cultured with or without lipopolysaccharide or CD40L. The culture supernatant and a tissue eluate were tested for donor-specific antibodies (DSA, by Luminex) and non-HLA antibodies (by antigen microarray). Supernatant from all 4 patients who had serum DSA at lung isolation was positive for DSA, with higher levels after CD40L stimulation, but only 2 of these had detectable DSA in lung eluate. Non-HLA antibody levels in CD40L-stimulated supernatant correlated with those in lung eluate but not with serum. Flow cytometry showed higher frequencies of activated lung B cells, and immunofluorescence showed more IgG-positive plasma cells and greater IL-21 expression in lymphoid aggregates with local antibodies. The authors conclude that DSA and non-HLA antibodies can be produced within activated B cell-rich lung allografts. The study is a small tissue-based laboratory study and does not show a clinical effect. The authors include Tereza Martinu, Stephen Juvet and Shaf Keshavjee.
Details
- doi
- 10.1016/j.healun.2024.07.019
- pmid
- 39097215
- authors
- Miyamoto E, Vosoughi D, Wang J, Al-Refaee J, Berra G, Daigneault T, Duong A, Joe B, Moshkelgosha S, Keshavjee S, Tinckam K, Hwang D, Chruscinski A, Juvet S, Martinu T
- journal
- The Journal of Heart and Lung Transplantation
- year
- 2025
- volume
- 44
- issue
- 1
- pages
- 105-117
Sources and links
- Publisher record via DOI (primary)
- PubMed 39097215
Related
Links from this record
- supports: Humoral (antibody-mediated) alloimmunity
- related to: Autoantibodies
- coauthor: Tereza Martinu
- coauthor: Stephen Juvet
- affiliation named in PubMed record (Toronto Lung Transplant Program): Toronto Lung Transplant Program (University Health Network)
Linked from (derived)
- supports: HLA donor-specific antibody and non-HLA antibody testing
- author: Shaf Keshavjee
Known gaps in this record
- full text not read (abstract only)
- funding statement and grant numbers
- full author affiliations (only the PubMed affiliation text was checked for named centres)
Information resource only. Not medical advice. Not a substitute for the care of the patient's transplant team.